Summary

A Research Technician III position is available at the Center for Advanced Innate Cell Therapy within the Texas Children’s Cancer Center. This position will be part of an interdisciplinary team of laboratory and clinical investigators developing novel forms of cancer immunotherapy. Duties  include cell culture, molecular cloning, PCR, T cell transduction, flow cytometry, in vitro functional assays of immune effector cells, and measuring antitumor activity in mice. We offer outstanding state-of-the-art, recently expanded laboratory facilities and a highly interactive work environment. The ideal candidate will have prior hands-on experience with cell culture, DNA/RNA processing, molecular cloning, gene transduction, flow cytometry, and handling mice. 

Job Duties

• Performs expansion of primary lymphocytes in culture.
• Performs lentivirus and retrovirus production.
• Performs retroviral and lentiviral transduction of cultured cells.
• Performs immunoflourescent staining and runs flow cytometry analysis.
• Analyses flow cytometry data using FlowJo software. 
• Performs T and/or NKT cell culture and measures their antitumor activity.
• Measures cytokine production using ELISA or Luminex assays.
• Isolates RNA and DNA from samples and processes samples for single cell RNA sequencing.
• Performs molecular cloning and PCR.
• Performs cell therapy experiments in mice.
• Measures BLI in mice.
• Processes peripheral blood, isolates mononuclear cells and specific cell subsets.
• Keeps laboratory records and mice inventory.
• Organizes lab space and keeps track of lab reagents and materials.
• Documents results of experiments and prepares reports.
• Generates reports summarizing experimental results.
• Performs other job related duties as assigned.

Minimum Qualifications

• Bachelor's degree in a Basic Science or related field. Four years of related experience may substitute for degree requirement.
• One year of relevant experience.

Preferred Qualifications

• Master's degree in basic science or a related filed.
• Three years of relevant experience that may include relevant laboratory work while in college.