Summary

The lab is seeking a highly motivated Research Technician II to support ongoing research efforts across two collaborative laboratories. 
• The Sartain Lab focuses on complement activation, endothelial dysfunction, microvascular thrombosis, and inflammatory activation pathways.
• The Yee Lab focuses on VWF structure-function, serine protease inhibition, protein–protein interaction mapping, and endothelial heterogeneity.
This role will be ideal for technician who is excited by cross-disciplinary science, interested in learning new experimental approaches, and participating in projects that range from molecular biochemistry to in vivo disease modeling. This is a part-time position, 10 hours/week. 

Job Duties

• Performs routine laboratory experiments, that include mammalian cell culture of primary human endothelial cells, protein and plasma-based biochemical assays (ELISA, Western blot, immunoblotting, immunoprecipitation), generation, expression, and purification of recombinant proteins (training provided; may include bacterial, mammalian, or phage display–based systems depending on project).
• Performs complement activation and hemostasis assays.
• Works on immunofluorescence microscopy and image analysis.
• Conducts phage display–based protein interaction mapping (training provided).
• Supports for flow cytometry experiments.
• Participates in ongoing studies of patient-derived plasma samples and maintain sample biorepositories.
• Prepares reagents, maintains laboratory organization and equipment, and ensures safety compliance.
• Contributes to lab meetings by presenting data and discussing experimental design.
• Assists in mouse colony maintenance, genotyping, and thrombosis/endothelial injury models (training provided).
• Works with other researchers, scientists, and principal investigators (PIs) to discuss findings, troubleshoot experiments, and advance projects.
• Analyzes and interprets complex datasets using statistical software or other specialized tools to draw conclusions.
• Compiles data into reports or presentations to communicate findings to other researchers, scientists, and principle investigators (PIs).
• Performs other job-related duties as assigned.

Minimum Qualifications

• Bachelor's degree in a Basic Science or related field. Four years of related experience may substitute for degree requirement.
• No experience required.

Preferred Qualifications

• Bachelor’s degree in Biology, Biochemistry, Molecular/Cell Biology, Biomedical Engineering, or a related field.
• Prior laboratory experience (course-based or research-based) preferred; all needed technical skills will be trained.
• Strong attention to detail, willingness to learn, and ability to work as part of a team.
• Experience in any of the following is beneficial, but not required:
  • Cell culture.
  • Western blot, ELISA, immunofluorescence.
  • Molecular cloning or PCR.
  • Flow cytometry sample prep.
  • Protein expression/purification.
  • Mouse handling / genotyping.
  • Analysis software: ImageJ, Prism, or NGS-based workflows.