Title:  Research Assistant II

Division:  Biochemistry
Schedule:  Monday - Friday 8:00 a.m - 5:00 p.m
Work Location:  Houston, TX
Salary Range:  $40,785 - $55,000
FLSA Status:  Exempt
Requisition ID:  7404


The Therapeutic Innovation Center is seeking talented cell and molecular biologists to work in an interdisciplinary team environment on early therapeutic and drug discovery projects. As our key focus is on diseases of gene regulation, with an emphasis on cancer, this position will involve the study of phenotypes associated with target perturbation in engineered cancer cell line and mouse models. Candidates will be expected to plan, execute and analyze assigned experiments with minimal supervision and to be able to troubleshoot as needed.

Job Purpose

Performs responsible research work of a complex nature that requires establishing test methods and procedures as well as planning, completing, and occasionally supervising limited research projects in accordance with general plans approved by the supervisor.

Job Duties

  • Plans and executes experimental protocols in mammalian cell culture including lentiviral production, cell growth/death assays and maintaining cell stocks.
  • Plans and executes standard molecular biology protocols including subcloning (Gateway, Topo, etc), qRT-PCR, PCR, DNA/RNA/protein isolation from cells and tissues.
  • Evaluates and analyzes data associated with assigned experiments.
  • Clearly documents and communicates (oral and written) experimental results and progress to principal investigators.
  • Assists in the preparation of laboratory reports.
  • Works collaboratively and effectively in a highly dynamic, multi-disciplinary environment.
  • Cleans and maintains laboratory equipment, supplies and work areas.
  • Handle biohazards or risks for potential job-related injury, such as those found in an ordinary laboratory environment.

Minimum Qualifications

  • Bachelor's degree in a Basic Science or a related field. 
  • Three years of relevant experience is required.

Preferred Qualifications

  • Master's degree in a related field.
  • Experience with mammalian cell culture, experience with a variety of cancer and/or normal cell types and growth conditions including meticulous sterile technique and attention to detail.
  • Experience with genetic perturbation techniques including RNAi and CRISPR/CAS9 technologies, lentiviral/retroviral production and stable cell line generation/maintenance.
  • Experience with handling mice and general animal care techniques including genotyping, tumor measurement and IP injections.
  • Experience with the following techniques: transfection/electroporation, DNA/RNA/protein extraction, western blot, qPCR, and standard subcloning (restriction digest based, Gateway, Topo etc).





Baylor College of Medicine is an Equal Opportunity/Affirmative Action/Equal Access Employer.